A comprehensive and immersive workshop on “Induced Pluripotent Stem Cells (iPSCs) and CRISPR-Based Genome Editing” was organized to equip researchers, postgraduate students, and professionals in biotechnology and life sciences with a robust understanding of advanced techniques in regenerative medicine and genetic engineering. The event was structured to bridge the gap between theoretical frameworks and real-world applications in stem cell biology and genome editing, fostering both conceptual clarity and hands-on proficiency. Forty-three doctoral students from all over the country participated in the workshop.
The workshop commenced with an in-depth exploration of iPSCs and organoids, beginning with the process of reprogramming differentiated somatic cells into pluripotent stem cells using key transcription factors, namely Oct4, Sox2, Klf4, and c-Myc. This session highlighted the significance of iPSCs in modeling human diseases, facilitating high-throughput drug screening, and enabling regenerative therapies, while also stressing their ethical superiority over embryonic stem cells. Participants were introduced to cutting-edge advancements in culturing iPSCs, optimizing differentiation protocols for lineage-specific cell types, and implementing rigorous quality control to ensure reproducibility and functional fidelity.
Building upon this foundation, the workshop transitioned to the CRISPR-Cas9 genome editing system. Detailed lectures were delivered on the molecular basis of CRISPR technology, including the design of guide RNAs, mechanisms of Cas9-mediated DNA cleavage, and the cellular DNA repair pathways—homology-directed repair (HDR) and non-homologous end joining (NHEJ). Through case studies, the application of CRISPR in correcting pathogenic mutations in iPSC-derived cells was demonstrated, underscoring its translational potential in therapeutic development.
Interactive hands-on sessions formed a core component of the workshop, allowing participants to engage in iPSC culture and maintenance, CRISPR-mediated gene editing, and subsequent genotyping and validation of edited cells. Participants also gained exposure to bioinformatics tools essential for CRISPR experiment design, including off-target prediction and analysis, enhancing their computational skill set.
To consolidate learning, a chalkboard discussion was conducted, encouraging attendees to collaboratively outline workflows and critical considerations in iPSC and genome editing research. This reflective session aimed to reinforce understanding and foster problem-solving approaches in experimental planning.
Parallel expert talks provided valuable insights into the broader significance of iPSC and CRISPR technologies in addressing intricate biological questions and translating discoveries into real-world solutions, particularly in the domains of precision medicine and agricultural biotechnology.
The workshop was designed and executed by Dr. Narasimha Swamy Telugu, Max Delbruck Centre, Germany and Dr. Prasad Tammineni, Department of Animal Biology, School of Life Sciences, University of Hyderabad. It was funded by Institution of Eminence, UoH, The DBT-India Alliance and the Department of Animal Biology. The coordinator, Skill Development Centre, thanked Dr. Narasimha Swamy Telugu, Dr. Prasad Tammineni and Dr. Kota Arun Kumar for all the supported extended.
Overall, the workshop served as a dynamic platform for knowledge dissemination and skill development, promoting innovation and collaboration in the rapidly evolving fields of stem cell research and genome engineering.